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KMID : 0380620110430060702
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 6 p.702 ~ p.710
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Determination of Aflatoxins in Nuts, Their Products and Dried Fruits Using Rapid Resolution Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Choi Su-Jeong
Park Ju-Sung
Jung So-Young
Son Yeo-Joon
Lee Yun-Jeong
Kim Mi-Sun
Park So-Hyun
Lee Sang-Me
Chae Young-Zoo
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Abstract
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The occurrence of aflatoxins B1, B2, G1 and G2 in nuts, their products and dried fruits was investigated. Samples were collected from local markets in Seoul and analyzed by rapid resolution liquid chromatography coupled with tandem mass spectrometry using an immunoaffinity column. The chromatography method was validated for assay of aflatoxins using linearity, accuracy, precision and limit of detection and quantification. The linearity in the concentration ranged from 0.10 to 20 μg/kg with R2>0.9999. Sample recoveries ranged from 71.1 to 97.2% with relative standard deviations below 4.5% for spiking levels from 1 to 10 μg/kg. The limits of detection ranged between 0.02 and 0.05 μg/kg and the limits of quantification ranged between 0.05 and 0.10 μg/kg. The levels of aflatoxin B1, B2, G1 and G2 in nuts, their products and dried fruits were B1 0.10 to 9.94 μg/kg, B2 0.08 to 1.54 μg/kg, G1 0.04 to 3.21 μg/kg and G2 0.06 to 0.14 μg/kg.
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KEYWORD
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aflatoxins, RRLC-MS/MS, nuts, dried fruits, validation
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